Laboratory Technician – Advanced Omics Platforms
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Localização: Botton-Champalimaud Pancreatic Cancer Center
Entidade Empregadora: Champalimaud Foundation
Regime: Tempo Inteiro
Posição: Research Técnico de Investigação
Início da Candidatura: 24 Abr. 2026

Offer Description

We are looking for a Laboratory Technician to be part of the Advanced Omics Platforms (AOP) of Botton-Champalimaud Pancreatic Cancer Centre, to provide technical services to support the current operation.

Sample Processing: Process human pancreatic tissues and patient-derived organoids into high-quality single-cell or single-nucleus suspensions using

mechanical and enzymatic dissociation protocols.

Quality Control: Perform rigorous quality control on isolated cells using techniques such as Flow Cytometry/FACS, Trypan Blue staining, and automated or manual cell counters.

Sequencing Preparation: Load single-cell suspensions onto the 10x Genomics Chromium Controller to generate barcoded suspensions ready for library generation and sequencing on the Illumina NextSeq 2000 instrument.

Protocol Execution: Perform laboratory protocols in accordance with Standard Operating Procedures (SOPs) to produce reliable and precise data for scientific research.

Sample Management: Handle fresh and archival samples (fresh-frozen and FFPE) from patient material and perform data entry/tracking using Laboratory Information Management Systems (LIMS).

Maintenance & Compliance: Ensure the cleanliness of the AOP lab space to comply with Quality System Regulation requirements and help maintain laboratory stocks of kits and reagents.

Collaboration: Develop positive relationships with internal teams, resolve technical issues efficiently, and participate in routine lab meetings to contribute to research planning.

STANDARDS OF PERFORMANCE

Proactively looking for opportunities to improve service provision and subsequent delivery on the improvements identified.

All individuals are expected to be flexible in undertaking the duties and responsibilities attached to their job and may be asked to perform other duties which reasonably correspond to the general character of their job and their level of responsibility.

All individuals are expected to maintain personal and professional development to meet the changing demands of the job and participate in appropriate learning and development activities.

Requirements

Research Field: Biological sciences

Education Level: Master Degree or equivalent

Years of Research Experience: 1 - 4

Skills / Qualifications

Essential Qualifications & Experience

Education: PhD or other post-graduate level qualification in life or medical sciences.

Languages: Proficient in both English and Portuguese.

Technical Skills: Significant experience with a variety of cell biology techniques including:

• Expertise in aseptic technique, tissue dissection, and the isolation of high-quality cells and/or nuclei from human tissues that are either fresh frozen or FFPE.
• Proficiency in viability counting and Flow Cytometry/FACS.
• Ability to follow SOPs and experimental workflows accurately and efficiently.

Soft Skills: Strong communication, organizational and IT skills.
Desirable Qualifications

• Single-cell sequencing experiments using the 10x Genomics Chromium Controller using either Flex or EpiMultiome.
• Experience with other 10x Genomics platforms such as the VisiumCytAssist and Xenium.
• Experience with Next-generation library workflows such as ATAC-seq.
• Dataentry and sampletracking using LIMS and document management systems.

Specific Requirements

KEY SKILLS

• Leadership
• Problem solving
• Flexibility